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KMID : 0939920070390010030
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대한암학회지
2007 Volume.39 No. 1 p.30 ~ p.36
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Analysis of Cellular Changes Resulting from Forced Expression of Dickkopf-1 in Hepatocellular Carcinoma Cells
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Kwack Mi-Hee
Hwang Sun-Young
Jang In-Seok
Im Sang-Uk
Kim Jin-O
Kim Moon-Kyu
Kim Jeong-Chul
Sung Young-Kwan
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Abstract
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Purpose:Recent studies have shown that Dickkopf-1 (DKK-1) is overexpressed in some tumors, including hepatocellular carcinoma. However, the role of increased DKK-1 in these tumors is not known. In this study, the DKK-1 expression in hepatocellular carcinoma (HCC) cell lines was evaluated and the effect of DKK-1 overexpression in HCC cell lines was studied.
Materials and Methods:The expression of DKK-1 in hepatocellular carcinoma cell lines was evaluated by RT-PCR. Stable cell lines that overexpressed DKK-1 were established. Cell growth, adhesion, migration and invasion assays were performed.
Results:RT-PCR analysis showed that 5 out of 8 HCC cell lines expressed DKK-1. The forced expression of DKK-1 suppressed the growth of cells and increased the population of cells in the sub-G1 phase. In addition, DKK- 1 reduced the cellular adhesion capacity to collagen type I and fibronectin, and it increased migratory capacity. However, overexpression of DKK-1 did not increase the invasion capacity of the HCC cell line.
Conclusion:Collectively, our data suggest that overexpression of DKK-1 affects the biology of HCC cells.
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KEYWORD
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Apoptosis, Cell adhesion, Cell line, Dickkopf-1, Hepatocellular carcinoma
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